Journal: PLoS ONE

Article Title: In Vitro Activity of Sodium New Houttuyfonate Alone and in Combination with Oxacillin or Netilmicin against Methicillin-Resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0068053

Figure Lengend Snippet: Susceptibility of MRSA strains to SNH and eight antibiotics.

Article Snippet: Kill kinetics of SNH was determined by time-kill experiments for five MRSA strains (three clinical isolates MRSA 5–20, MRSA 6–29 and MRSA 8–36 and two reference strains ATCC 33591 and Mu50) according to the method described by Verma et al. with slight modifications .

Techniques:

Journal: PLoS ONE

Article Title: In Vitro Activity of Sodium New Houttuyfonate Alone and in Combination with Oxacillin or Netilmicin against Methicillin-Resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0068053

Figure Lengend Snippet: Effects of SNH in combination with antibiotics against 12 MRSA strains in checkerboard assay.

Article Snippet: Kill kinetics of SNH was determined by time-kill experiments for five MRSA strains (three clinical isolates MRSA 5–20, MRSA 6–29 and MRSA 8–36 and two reference strains ATCC 33591 and Mu50) according to the method described by Verma et al. with slight modifications .

Techniques:

Journal: PLoS ONE

Article Title: In Vitro Activity of Sodium New Houttuyfonate Alone and in Combination with Oxacillin or Netilmicin against Methicillin-Resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0068053

Figure Lengend Snippet: A) MRSA 5–20, 1/2×MIC SNH-1/64×MIC OXA (MIC of SNH = 32 µg/mL, MIC of OXA = 128 µg/mL); B) MRSA 6–29, 1/2×MIC SNH-1/64×MIC OXA (MIC of SNH = 32 µg/mL, MIC of OXA = 256 µg/mL); C) MRSA 8–36, 1/2×MIC SNH-1/128×MIC OXA (MIC of SNH = 32 µg/mL, MIC of OXA = 512 µg/mL); D) ATCC 33591 1/2×MIC SNH-1/64×MIC OXA (MIC of SNH = 32 µg/mL, MIC of OXA = 256 µg/mL); E) Mu 50, 1/2×MIC SNH-1/128×MIC OXA (MIC of SNH = 64 µg/mL, MIC of OXA = 512 µg/mL); ▪, GC, growth control; ▴, SNH; ◊, OXA; ○, combination of SNH and OXA.

Article Snippet: Kill kinetics of SNH was determined by time-kill experiments for five MRSA strains (three clinical isolates MRSA 5–20, MRSA 6–29 and MRSA 8–36 and two reference strains ATCC 33591 and Mu50) according to the method described by Verma et al. with slight modifications .

Techniques: Control

Journal: PLoS ONE

Article Title: In Vitro Activity of Sodium New Houttuyfonate Alone and in Combination with Oxacillin or Netilmicin against Methicillin-Resistant Staphylococcus aureus

doi: 10.1371/journal.pone.0068053

Figure Lengend Snippet: A) MRSA 5–20, 1/2×MIC SNH-1/4×MIC NET (MIC of SNH = 32 µg/mL, MIC of NET = 16 µg/mL); B) MRSA 6–29, 1/2×MIC SNH-1/2×MIC NET (MIC of SNH = 32 µg/mL, MIC of NET = 8 µg/mL); C) MRSA 8–36, 1/2×MIC SNH-1/8×MIC NET (MIC of SNH = 32 µg/mL, MIC of NET = 64 µg/mL); D) ATCC 33591 1/2×MIC SNH-1/4×MIC NET (MIC of SNH = 32 µg/mL, MIC of NET = 4 µg/mL); E) Mu 50, 1/2×MIC SNH-1/4×MIC NET (MIC of SNH = 64 µg/mL, MIC of NET = 16 µg/mL); ▪, GC, growth control; ▴, SNH; ▽, NET; ○, combination of SNH and NET.

Article Snippet: Kill kinetics of SNH was determined by time-kill experiments for five MRSA strains (three clinical isolates MRSA 5–20, MRSA 6–29 and MRSA 8–36 and two reference strains ATCC 33591 and Mu50) according to the method described by Verma et al. with slight modifications .

Techniques: Control

Journal: International Journal of Microbiology

Article Title: Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

doi: 10.1155/2014/650328

Figure Lengend Snippet: 2% Agarose gel electrophoresis analysis of PCR amplification products of mecA gene of 310 bp, extracted from S. aureus . Lane1: negative control (no DNA template); lane 2: positive control ( mecA positive strain ATCC 33591); lanes 3–6: methicillin-resistant S. aureus (MRSA); lane M: DNA molecular size marker (100 bp ladder).

Article Snippet: A standard MSSA strain (ATCC 25923) and a MRSA strain (ATCC 33591) were used as control.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, Positive Control, Marker

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: The insulin-like growth factor receptor (IGF-1R) inhibitor PQ401 exhibits bactericidal activity against S. aureus strain MW2 with no detectable resistance development. (A) Chemical structure of PQ401. (B) MRSA-infected C. elegans was treated with 5 μg/ml PQ401, 10 μg/ml vancomycin (Van, positive control), and 0.1% DMSO (DMSO, negative control). Dead worms were stained with SYTOX Orange. (C) Exponential-phase S. aureus MW2 was treated with PQ401 or vancomycin for 4 h. The bacterial viability was measured at hourly intervals. The limitation of detection is 2 × 10 2 CFU/ml. Error bars denote SD ( n = 3). (D) Three attempts to develop MRSA resistance to PQ401 (SP 1, 2, and 3) and to ciprofloxacin (Cipro) over 25 days.

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Activity Assay, Infection, Positive Control, Negative Control, Staining

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: MIC of PQ401 against MRSA strains

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques:

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: Minimum bactericidal concentration of PQ401 against MRSA strains

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Concentration Assay

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: Proportion of each ionized form of PQ401 and their MICs against MRSA strain MW2 ^a

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques:

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: PQ401 has bactericidal potency and shows synergism with gentamicin against antibiotic-tolerant MRSA. Antibiotic-tolerant cells of MRSA MW2 were treated with 100× MIC vancomycin (Van), gentamicin (Gm), or ciprofloxacin (Cipro) (A); the indicated concentrations of PQ401 (B); or 10 μg/ml gentamicin (Gm) in combination with various concentrations of PQ401 for 4 h (C). CFU counts of viable cells were measured by serial dilution and plating on TSA plates. Results are shown as means ± SD ( n = 3).

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Serial Dilution

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: Antibacterial Effects of Various Types of Bee Products in Malaysia: A Systematic Review

doi: 10.21315/mjms2024.31.3.3

Figure Lengend Snippet: Overview characteristics of included studies

Article Snippet: Ng and Lim ( ) , Gelam honey , Staphylococcus aureus methicillin sensitive (MSSA) strains (ATCC 25923 and ATCC 6538) and methicillin-resistant (MRSA) strains (ATCC 33592 and ATCC 33591) , Agar well diffusion method , Zone of inhibition against tested bacteria MSSA ATCC 25923: 0.0–10.7 mm MSSA ATCC 6538: 0.0–10.0 mm MRSA ATCC 33592: 0.0–13.0 mm MRSA ATCC 33591: 0.0–13.7 mm.

Techniques: Bacteria, Diffusion-based Assay, Inhibition, Concentration Assay, Biofilm Production Assay, Microscopy, Disruption, Confocal Laser Scanning Microscopy, Imaging, Membrane, Northern Blot